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The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein. Western Blotting is an analytical technique used to detect specific proteins in a given sample.
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To study tumor relapse we initially looked for a mouse model in which tumors recur following their complete regression.
. - Western Blot - AbVideo - Support - Abnova. Since the effect of classic checkpoint blockade therapies are limited in most mouse models we used a combination of dendritic cell adjuvant with tumor-binding antibodies to elicit highly potent T-cell immunity Spitzer et al 2017. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide.
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